| Antibody |
EEA1 (rabbit monoclonal) |
Cell Signaling Technology |
Cat#3288 S, RRID:AB_2096811
|
IF (1:100) |
| Antibody |
Lysozyme (rabbit polyclonal) |
Thermo Fisher Scientific |
Cat#PA5-16668, RRID:AB_10984852
|
IF (1:100) |
| Antibody |
Rab3D (rabbit polyclonal) |
Synaptic systems |
Cat#107 303, RRID:AB_2253547
|
IF (1:100) |
| Antibody |
Rab7 (rabbit monoclonal) |
Cell Signaling Technology |
Cat#9367T, RRID:AB_1904103
|
IF (1:100) |
| Antibody |
LAMP-1 (rabbit polyclonal) |
Abcam |
Cat#ab62562RRID:AB_2134489
|
IF (1:100) |
| Antibody |
TGN46 (rabbit monoclonal) |
Thermo Fisher |
Cat#MA5-32532RRID:AB_2809809
|
IF (1:100) |
| Antibody |
VAMP8 (chicken polyclonal) |
University of Texas |
Prof Burton Dickey |
IF (1:500) |
| Antibody |
Calnexin (rabbit polyclonal) |
Abcam |
Cat#ab22595RRID:AB_2069006
|
IF (1:100) |
| Antibody |
Goat anti-Chicken IgY Alexa Fluor 555(goat polyclonal) |
Thermo Fisher Scientific |
Cat#A32932RRID:AB_2762844
|
IF (1:1000) |
| Antibody |
Goat anti-Rabbit IgG Alexa Fluor 488 (goat polyclonal) |
Thermo Fisher Scientific |
Cat#A-11008, RRID:AB_143165
|
IF (1:1000) |
| Antibody |
Goat anti-Rabbit IgG Alexa Fluor 647 (goat polyclonal) |
Thermo Fisher Scientific |
Cat#A-21244RRID:AB_2535812
|
IF (1:1000) |
| Chemical compound, drug |
Atropine |
Sigma-Aldrich |
Cat#A0257 |
550 µg/kg i.p. |
| Chemical compound, drug |
Telenzepine |
Sigma-Aldrich |
Cat#T122 |
550 µg/kg i.p. |
| Chemical compound, drug |
4-DAMP |
Sigma-Aldrich |
Cat#SML0255 |
550 µg/kg i.p. |
| Chemical compound, drug |
Tropicamide |
Tocris |
Cat#0909 |
100 µg/kg s.c. |
| Chemical compound, drug |
Carbamylcholine |
Sigma-Aldrich |
Cat#C4382 |
125 µg/kg s.c. |
| Chemical compound, drug |
FM 1–43FX |
Thermo Fisher Scientific |
Cat#F35355
|
50 µg/ml |
| Chemical compound, drug |
EGTA |
Sigma-Aldrich |
Cat#E3889 |
2 mM |
| Chemical compound, drug |
BAPTA-AM |
Sigma-Aldrich |
Cat#A1076 |
200 µM |
| Chemical compound, drug |
Xestospongin C |
Sigma-Aldrich |
Cat#X2628 |
22 µM |
| Chemical compound, drug |
Dynasore |
Sigma-Aldrich |
Cat#D7693 |
150 µM |
| Chemical compound, drug |
Dyngo 4a |
Selleck Chemicals |
Cat#S7163 |
120 µM |
| Chemical compound, drug |
Colchicine |
Tocris |
Cat#1364 |
100 µM |
| Chemical compound, drug |
Cytochalasin D |
Tocris |
Cat#1233 |
4 µM |
| Chemical compound, drug |
Ciliobrevin D |
Sigma-Aldrich |
Cat#250401 |
100 µM |
| Chemical compound, drug |
Dimethylenastron (DMEA) |
Tocris |
Cat#5261 |
10 µM |
| Chemical compound, drug |
8-Br-cADPr |
Sigma-Aldrich |
Cat#B5416 |
0.2 mg/kg i.p. |
| Chemical compound, drug |
Trans-Ned19 |
Tocris |
Cat#3954 |
5 mg/kg i.p |
| Chemical compound, drug |
LY294002 |
Sigma-Aldrich |
Cat#L9908 |
4 mg/kg i.p |
| Chemical compound, drug |
Diamidino-2-phenylindole (DAPI) |
Sigma-Aldrich |
Cat#D9542 |
1 µg/ml |
| Chemical compound, drug |
UEA1 Fluorescein |
Vector Laboratories |
Cat#FL-1061–2 |
10 µg/ml |
| Chemical compound, drug |
WGA Fluorescein |
Vector Laboratories |
Cat#FL-1021 |
10 µg/ml |
| Chemical compound, drug |
WGA Texas Red |
Sigma-Aldrich |
Cat#W21405
|
10 µg/ml |
| Chemical compound, drug |
Dextran tetramethylrodamine conjugate, lysine fixable, MW 10,000 |
Thermo Fisher Scientific |
Cat#D1817 |
12.5 mg/ml |
| Chemical compound, drug |
Dextran biotin conjugate, lysine fixable, MW 10,000 |
Thermo Fisher Scientific |
Cat#D1956 |
12.5 mg/ml |
| Chemical compound, drug |
Dextran Alexa 647 conjugate lysine fixable, MW 10,000 |
Thermo Fisher Scientific |
Cat#D22914
|
12.5 mg/ml |
| Chemical compound, drug |
Ovalbumin Texas Red conjugate |
Thermo Fisher Scientific |
Cat#O23021
|
12.5 mg/ml |
| Chemical compound, drug |
Ni DAB |
Vector Laboratories |
Cat#SK-4100, RRID:AB_2336382
|
|
| Commercial assay or kit |
ABC Elite kit |
Vector Laboratories |
Cat#PK-6100, RRID:AB_2336819
|
|
| Commercial assay or kit |
RNAscope fluorescent reagent kit v2 |
ACD, Bio-Techne |
Cat# 323100 |
|
| Sequence-based reagent |
Mm-Chrm1-O1-C2 |
ACD, Bio-Techne |
Cat#483441-C2 |
|
| Sequence-based reagent |
Mm-Chrm3 |
ACD, Bio-Techne |
Cat#437701 |
|
| Sequence-based reagent |
Mm-Chrm4-C2Chrm4 |
ACD, Bio-Techne |
Cat#410581-C2 |
|
| Species, species background (Mus musculus) |
Mouse: C57 Bl/6 J |
Jackson Laboratories |
Stock No: 000664, RRID:IMSR_JAX:000664
|
|
| Species, species background (Mus musculus) |
Mouse: Itgax YFP |
Jackson Laboratories |
Stock No: 008829RRID: IMSR_JAX:008829
|
|
| Species, species background (Mus musculus) |
Mouse: Cx3cr1 GFP |
Jackson Laboratories |
Stock No: 005582RRID: IMSR_JAX:005582
|
|
| Software, algorithm |
GraphPad Prism 7 |
GraphPad |
https://www.graphpad.com, RRID:SCR_002798
|
|
| Software, algorithm |
Amira |
Thermofisher |
http://www.fei.com/software/amira-3d-for-life-sciences/, RRID:SCR_007353
|
|
| Software, algorithm |
Zen |
Zeiss |
http://www.zeiss.com/microscopy/en_us/products/microscope-software/zen.html#introduction, RRID:SCR_013672
|
|
| Software, algorithm |
Fiji |
Schindelin et al., 2012
|
https://fiji.sc/, RRID:SCR_002285
|
|
| Software, algorithm |
AxioVision |
Zeiss |
http://www.zeiss.com/microscopy/en_de/products/microscope-software/axiovision-for-biology.html, RRID:SCR_002677
|
|
| Software, algorithm |
Imaris |
Bitplane |
http://www.bitplane.com/imaris/imaris RRID:SCR_007370
|
|
| Software, algorithm |
ImageWarp |
A&B Software |
http://www.imagewarp.com/
|
|
