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. 2021 Nov 9;2021:4132488. doi: 10.1155/2021/4132488

Figure 3.

Figure 3

STAT3 activates transcription activity of miR-19a in the hypoxia-exposed CRC cells: (a) possible upstream regulators of miR-19a predicted using TransmiR v2.0; (b) five segments of miR-19a promoter divided from the distal side to near side; (c) luciferase activity of the miR-19a promoter segments in HEK293T cells after hypoxia exposure (∗p < 0.05, two-way ANOVA); (d) binding relationship between STAT3 and the miR-19a promoter validated using a ChIP-qPCR assay (∗p < 0.05, two-way ANOVA); (e) mRNA expression of STAT3 in tumor tissues and the paracancerous tissues examined by RT-qPCR; (f) a positive correlation between STAT3 and miR-19a in CRC tumor tissues (∗p < 0.05, Pearson's correlation analysis); (g) phosphorylation of STAT3 in HCT116 and SW480 cells after hypoxia exposure examined by western blot analysis. Data were presented as the mean ± SD from three independent experiments.