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. 2021 Nov 9;2021:4132488. doi: 10.1155/2021/4132488

Figure 4.

Figure 4

miR-19a mediates the PI3K/AKT signaling pathway in the hypoxia-exposed CRC cells: (a) downstream mRNAs of miR-19a predicted using the StarBase system; (b) transfection efficacy of miR-19a mimic examined using RT-qPCR (∗p < 0.05, two-way ANOVA); (c) activation of PI3K/AKT and Wnt/β-catenin signaling pathways in HCT116 and SW480 cells after miR-19a mimic transfection examined by western blot analysis (∗p < 0.05, two-way ANOVA); (d, e) expression of miR-19a and STAT3 in HCT116 and SW480 cells after si-STAT3 or si-STAT3+miR-19a mimic transfection examined by RT-qPCR (∗#p < 0.05, two-way ANOVA; ∗compared to hypoxia+STAT3-NC; #compared to si-STAT3+miR-19a control group); (f) phosphorylation of PI3K and AKT in cells examined by western blot analysis (∗#p < 0.05, two-way ANOVA; ∗compared to hypoxia+STAT3-NC; #compared to si-STAT3+miR-19a control group). Data were presented as the mean ± SD from three independent experiments.