Table 1.
Primer sequences for RT-qPCR.
| Gene | Primer sequence (5′-3′) |
|---|---|
| miR-19a | F: CTGGAGTGTGCAAATCTATGC |
| R: GTGCAGGGTCCGAGGT | |
|
| |
| U6 | F: GCTTCGGCAGCACATATACTAAAAT |
| R: CGCTTCACGAATTTGCGTGTCAT | |
|
| |
| STAT3 | F: CATCCTGAAGCTGACCCAGG |
| R: TATTGCTGCAGGTCGTTGGT | |
|
| |
| PTEN | F: ACCAGGACCAGAGGAAACCT |
| R: GCTAGCCTCTGGATTTGACG | |
|
| |
| GAPDH | F: CTGACTTCAACAGCGACACC |
| R: GTGGTCCAGGGGTCTTACTC | |
RT-qPCR: reverse transcription quantitative polymerase chain reaction; miR-19a: microRNA-19a; STAT3: signal transducer and activator of transcription 3; PTEN: phosphatase and tensin homolog; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; F: forward; R: reverse.