Table 4.
Effects of bisdemethoxycurcumin on the activities of the mitochondrial respiratory complexes (I-V) of jejunum and ileum in LPS-treated broilers.
| Items | LPS (-) | LPS (+) | SEM | P | ||||
|---|---|---|---|---|---|---|---|---|
| CON 2 | BIS | LPS | L-BIS | Diet | Stress | D × S | ||
| Jejunum | ||||||||
| Complex I (μmol NADH/min/mg protein) | 9.90a | 9.85a | 6.16b | 8.83a | 0.237 | 0.014 | <0.001 | 0.011 |
| Complex II (μmol NADH/min/mg protein) | 6.76a | 6.64a | 4.46b | 6.28a | 0.215 | 0.066 | 0.007 | 0.038 |
| Complex III (μmol cytochrome c/min/mg protein) | 16.66 | 17.12 | 11.51 | 14.54 | 0.528 | 0.118 | 0.002 | 0.242 |
| Complex IV (μmol CoQH2/min/mg protein) | 28.98a | 28.49a | 17.12b | 24.19a | 0.633 | 0.019 | <0.001 | 0.009 |
| Complex V (μmol DCPIP/min/mg protein) | 44.95 | 47.27 | 32.62 | 42.05 | 1.266 | 0.034 | 0.003 | 0.180 |
| Ileum | ||||||||
| Complex I (μmol NADH/min/mg protein) | 10.12 | 9.98 | 6.87 | 8.19 | 0.132 | 0.042 | <0.001 | 0.130 |
| Complex II (μmol NADH/min/mg protein) | 6.29 | 6.52 | 4.29 | 5.85 | 0.142 | 0.006 | <0.001 | 0.320 |
| Complex III (μmol cytochrome c/min/mg protein) | 20.00a | 19.27a | 12.96b | 18.59a | 0.412 | 0.009 | <0.001 | 0.001 |
| Complex IV (μmol CoQH2/min/mg protein) | 27.56ab | 28.10a | 20.87c | 24.61b | 0.374 | 0.011 | <0.001 | 0.048 |
| Complex V (μmol DCPIP/min/mg protein) | 42.23 | 44.76 | 28.36 | 36.84 | 0.733 | 0.002 | <0.001 | 0.059 |
CON: broiler chickens fed a basal diet; BIS: broiler chickens fed a basal diet with 150 mg/kg bisdemethoxycurcumin; LPS: broiler chickens fed a basal diet with LPS injection; L-BIS: broiler chickens fed a basal diet with LPS injection and 150 mg/kg bisdemethoxycurcumin. Diet: 0 or 150 mg/kg bisdemethoxycurcumin treatment; stress: saline or LPS challenge; D × S: interaction between bisdemethoxycurcumin treatment and LPS challenge. Data were expressed as means and SEM, n = 8 replicates per treatment and 10 birds per replication. Means within a row with no common superscript differ P < 0.05.