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The effect of limonin on IL-1β-activated ECM degradation in chondrocytes. (a–e) The protein expression of Aggrecan, Collagen II, ADAMTS5, and MMP13 in chondrocytes was evaluated by western blot assay. (f–h) The protein expression of Collagen II and MMP13 was evaluated by cell immunofluorescence (scale bar: 10 μm) and ImageJ. The results in the figures are represented as averages ± S.D. Significant differences among various groups are represented as ∗p < 0.05, ∗∗p < 0.01; n = 3.
