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(A) Rh30 cell clones expressing PAX3-KRAB (3B, 3D, and 3N) show reduced colony formation in soft agar compared with vector clones (1B and 1C) or parental Rh30 cells. An equal number of the indicated Rh30 clones (5 × 104 cells) was suspended in 0.4% agarose, and colonies were photographed at 14 to 21 days. (B) Soft-agar colonies of Rh30 cell clones (vector 1B versus PAX3-KRAB-3D). Phase-contrast magnification, ×20.
