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. 2021 Nov 3;12:763912. doi: 10.3389/fimmu.2021.763912

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Copyright © 2021 Vatzia, Allen, Manjegowda, Morris, McNee, Martini, Kaliath, Ulaszewska, Boyd, Paudyal, Carr, Chrun, Maze, MacLoughlin, van Diemen, Everett, Lambe, Gilbert and Tchilian

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IFNγ ELISpot responses in PBMC. IFNγ secreting spot-forming cells (SFC) were enumerated during the time course following stimulation with a pool of peptides covering (A) NP, (B) M1, and (C) NA proteins or (D) pH1N1 and (E) H3N2 viruses. The arrows below D0 and D28 indicate the challenge of the pigs with pH1N1 and the immunization with ChAdOx2-NPM1-NA, respectively. The C group was not immunized. Each symbol represents an individual animal, the top of the bar represents the mean, and the line the standard error (SEM) except for graph (B), where the top of the bar indicates the median and the line the interquartile range. Significant statistical differences are listed in Table 2, 3. .