Figure 6.

T-cell cytokine responses in the spleen and nasal turbinates. (A–D) Spleen and (E–H) nasal turbinates were collected at 4 weeks post-immunization. Cryopreserved cells were thawed and stimulated with (A, C, E, G) pH1N1 or (B, D, F, H) H3N2, and IFNγ, TNF, and IL-2 cytokine secretions were measured in CD4 and CD8 T cells by intracellular cytokine staining. Each symbol represents an individual animal; the top of the bar indicates the mean and the line the standard error mean (SEM). Two-Way ANOVA and Bonferroni multiple comparisons tests were used to compare responses between groups, and asterisks indicate significant differences (*p < 0.05).