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Antibodies used for the intracellular cytokine staining.
| Antigen | Clone | Isotype | Fluorochrome | Source of primary Ab | Details of secondary Ab |
|---|---|---|---|---|---|
| CD4 | 74-12-4 | IgG2b | PerCP-Cy5.5 | BD Biosciences | |
| CD8b | PPT23 | IgG1 | FITC | Bio-Rad Laboratories | |
| TNF | MAb11 | IgG1 | BV421 | BioLegend | |
| IFNγ | P2G10 | IgG1 | APC | BD Biosciences | |
| IL-2 | A150D 3F1 2H2 | IgG2a | PE-Cy7 | Thermo Fisher | Rat anti-mouse, IgG2a, BioLegend |
