TABLE 4.

List of synthetic DNA used in genetic engineering.

Sequence name	Sequence	Purpose
P BAD -rseA27-lacZ gBlock	Acc​tga​cgc​ttt​tta​tcg​caa​ctc​tct​act​gtt​tct​cca​TGA​GAC​AGA​TAG​TTT​TCC​GAA​CTA​TTG​AGT​CCC​TCC​CGG​AAG​ATT​TAC​GCA​TGG​CAA​TAA​CCT​TGC​GGG​AGC​TGG​ATG​GCC​TGA​GCT​ATG​AAG​AGA​TAG​CCG​CTA​TCA​TGG​ATT​GTC​CGG​TAG​GTA​CGG​TGC​GTT​CAC​GTA​TCT​TCC​GAG​CGA​GGG​AAG​CTA​TTG​ATA​ACA​AAG​TTC​AAC​CGC​TTA​TCA​GGC​GTT​GAC​GAT​AGC​GGG​ATA​CTG​GAT​AAG​GGT​ATT​AGG​Cat​gCA​GAA​AGA​ACA​ACT​TTC​CGC​TTT​AGC​CGT​CGT​TTT​ACA​ACG​TCG​TGA​CTG​GGA​AAA​CCC​TGG​CG	used as a template for amplification of the recombination substrate for creation of P BAD -rseA27-lacZ translational fusion
P BAD -rseA27cm1-lacZ gBlock	Acc​tga​cgc​ttt​tta​tcg​caa​ctc​tct​act​gtt​tct​cca​TGA​GAC​AGA​TAG​TTT​TCC​GAA​CTA​TTG​AGT​CCC​TCC​CGG​AAG​ATT​TAC​GCA​TGG​CAA​TAA​CCT​TGC​GGG​AGC​TGG​ATG​GCC​TGA​GCT​ATG​AAG​AGA​TAG​CCG​CTA​TCA​TGG​ATT​GTC​CGG​TAG​GTA​CGG​TGC​GTT​CAC​GTA​TCT​TCC​GAG​CGA​CCC​AAG​CTA​TTG​ATA​ACA​AAG​TTC​AAC​CGC​TTA​TCA​GGC​GTT​GAC​GAT​AGC​GGG​ATA​CTG​GAT​AAG​GGT​ATT​AGG​Cat​gCA​GAA​AGA​ACA​ACT​TTC​CGC​TTT​AGC​CGT​CGT​TTT​ACA​ACG​TCG​TGA​CTG​GGA​AAA​CCC​TGG​CG	used as a template for amplification of the recombination substrate for creation of P BAD -rseA27(cm1)-lacZ translational fusion
P BAD -rseA27cm3-lacZ gBlock	Acc​tga​cgc​ttt​tta​tcg​caa​ctc​tct​act​gtt​tct​cca​TGA​GAC​AGA​TAG​TTT​TCC​GAA​CTA​TTG​AGT​CCC​TCC​CGG​AAG​ATT​TAC​GCA​TGG​CAA​TAA​CCT​TGC​GGG​AGC​TGG​ATG​GCC​TGA​GCT​ATG​AAG​AGA​TAG​CCG​CTA​TCA​TGG​ATT​GTC​CGG​TAG​GTA​CGG​TGC​GTT​CAC​GGA​GTG​GGC​GAG​CGA​GGG​AAG​CTA​TTG​ATA​ACA​AAG​TTC​AAC​CGC​TTA​TCA​GGC​GTT​GAC​GAT​AGC​GGG​ATA​CTG​GAT​AAG​GGT​ATT​AGG​Cat​gCA​GAA​AGA​ACA​ACT​TTC​CGC​TTT​AGC​CGT​CGT​TTT​ACA​ACG​TCG​TGA​CTG​GGA​AAA​CCC​TGG​CG	used as a template for amplification of the recombination substrate for creation of P BAD -rseA27(cm3)-lacZ translational fusion
P BAD -rseA-3XFLAG gBlock	AcctgacgctttttatcgcaactctctactgtttctccaTGAGACAGATAGTTTTCCGAACTATTGAGTCCCTCCCGGAAGATTTACGCATGGCAATAACCTTGCGGGAGCTGGATGGCCTGAGCTATGAAGAGATAGCCGCTATCATGGATTGTCCGGTAGGTACGGTGCGTTCACGTATCTTCCGAGCGAGGGAAGCTATTGATAACAAAGTTCAACCGCTTATCAGGCGTTGACGATAGCGGGATACTGGATAAGGGTATTAGGCatgCAGAAAGAACAACTTTCCGCTTTAATGGATGGCGAAACGCTGGATAGTGAGCTGCTTAACGAACTGGCTCATAACCCAGAAATGCAGAAAACCTGGGAAAGCTATCACTTAATCCGTGACTCAATGCGGGGTGATACTCCCGAGGTGCTCCATTTCGATATCTCTTCACGCGTGATGGCCGCCATTGAAGAAGAGCCAGTACGTCAACCGGCGACATTGATCCCGGAAGCCCAGCCTGCGCCGCATCAATGGCAGAAAATGCCATTCTGGCAGAAAGTACGTCCGTGGGCGGCACAGCTTACCCAAATGGGCGTAGCCGCATGCGTATCGCTTGCAGTTATCGTTGGCGTCCAGCACTATAATGGACAATCTGAAACGTCCCAGCAGCCCGAAACGCCGGTATTTAATACACTGCCGATGATGGGTAAAGCCAGCCCGGTAAGCCTGGGAGTACCTTCTGAAGCGACCGCAAACAATGGTCAACAGCAGCAGGTACAGGAGCAGCGTCGTCGCATTAATGCAATGTTGCAGGATTACGAACTGCAACGCCGACTCCACTCTGAACAGCTTCAGTTTGAGCAGGCGCAAACCCAGCAAGCCGCTGTACAGGTGCCAGGAATTCAAACTTTAGGAACGCAATCGCAGGATTACAAAGATCATGACGGGGACTACAAAGATCACGATATAGATTATAAAGATGACGATGACAAAtaaATTATAAAAATTGCCTGATACGCTGCGCTTATCAGGCCTA	used as a template for amplification of recombination substrate for creation of P BAD -rseA-3XFLAG
P BAD -rseAcm1-3XFLAG gBlock	AcctgacgctttttatcgcaactctctactgtttctccaTGAGACAGATAGTTTTCCGAACTATTGAGTCCCTCCCGGAAGATTTACGCATGGCAATAACCTTGCGGGAGCTGGATGGCCTGAGCTATGAAGAGATAGCCGCTATCATGGATTGTCCGGTAGGTACGGTGCGTTCACGTATCTTCCGAGCGACCCAAGCTATTGATAACAAAGTTCAACCGCTTATCAGGCGTTGACGATAGCGGGATACTGGATAAGGGTATTAGGCatgCAGAAAGAACAACTTTCCGCTTTAATGGATGGCGAAACGCTGGATAGTGAGCTGCTTAACGAACTGGCTCATAACCCAGAAATGCAGAAAACCTGGGAAAGCTATCACTTAATCCGTGACTCAATGCGGGGTGATACTCCCGAGGTGCTCCATTTCGATATCTCTTCACGCGTGATGGCCGCCATTGAAGAAGAGCCAGTACGTCAACCGGCGACATTGATCCCGGAAGCCCAGCCTGCGCCGCATCAATGGCAGAAAATGCCATTCTGGCAGAAAGTACGTCCGTGGGCGGCACAGCTTACCCAAATGGGCGTAGCCGCATGCGTATCGCTTGCAGTTATCGTTGGCGTCCAGCACTATAATGGACAATCTGAAACGTCCCAGCAGCCCGAAACGCCGGTATTTAATACACTGCCGATGATGGGTAAAGCCAGCCCGGTAAGCCTGGGAGTACCTTCTGAAGCGACCGCAAACAATGGTCAACAGCAGCAGGTACAGGAGCAGCGTCGTCGCATTAATGCAATGTTGCAGGATTACGAACTGCAACGCCGACTCCACTCTGAACAGCTTCAGTTTGAGCAGGCGCAAACCCAGCAAGCCGCTGTACAGGTGCCAGGAATTCAAACTTTAGGAACGCAATCGCAGGATTACAAAGATCATGACGGGGACTACAAAGATCACGATATAGATTATAAAGATGACGATGACAAAtaaATTATAAAAATTGCCTGATACGCTGCGCTTATCAGGCCTA	used as a template for amplification of recombination substrate for creation of P BAD -rseAcm1-3XFLAG