FIG. 3.

Variant RSSs and configuration-specific RJP. The sequence of each tested RSS is given, indicating matches to the canonical RSS in uppercase letters. Spacer sequences were changed according to the dictates of a given experiment. The 12a spacer is an arbitrary sequence as given previously (25). The 12b spacer is identical to the spacer of the RSS appearing in site 2, and the 12c spacer is a continuation of the CA repeat. The total number of recombinants typed as either site 1 or site 2 rearrangements is given for each of the SJΔ and Inv constructs. For experiments where the site 2 control was introduced on a separate plasmid, the values shown are calculated as described in Materials and Methods (a sample calculation is given in Fig. 4). The RJP is defined as the ratio of site 1 to site 2 recombinants. The ratio of inversion to SJΔ provides a means by which to compare the stage 2 impact of a different RSS variants. Superscript numbers: 1, cotransfection of p6131-SJΔno2 and the site 2 control plasmid (see Fig. 2C); 2, cotransfection of p6131-Invno2 and the site 2 control plasmid; 3, cotransfection of p6131-Inv(506) (in which the distance between sites 1 and 3 was increased from 279 to 506 bp) and the site 2 control plasmid; and 4, cotransfection of p6131-CJΔ(546) (in which the distance between sites 1 and 3 was increased from 319 to 546 bp) and the site 2 control plasmid.