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. 2021 Nov 16;11:22341. doi: 10.1038/s41598-021-01696-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Monoclonal antibody concentration–response curves in vitro. The concentration-dependent ADAMTS13 inhibitory activity of human (A) and mouse (B) antibodies was determined by incubating normal pooled plasma with 2-fold serial dilutions of the antibodies ranging from 25 µg/mL to 0.20 µg/mL in triplicate. The values of dose 0 control were also measured to calculate the residual ADAMTS13 activity of each antibody. The horizontal axis is the concentration of antibodies used in these tests. The vertical axis is the residual plasma ADAMTS13 activity relative to the values of dose 0 control.