Figure 2.
Nasal colonization and bacteremia in the mono-infection model. Mice were intranasally inoculated with different two doses [1 × 105 for (A, B) and 1 × 107 CFU/mouse for (C, D)] of S. pneumoniae without anesthesia on day 0. Nasal lavage and blood were collected on day 2 for counting colonies. Each symbol represents individual data. For low-dose groups (A, B), 5 of TRPV1 KO, 5 of TRPV4 KO, and 5 of wild-type mice were used. For high-dose groups (C, D), 14 of TRPV1 KO, 20 of TRPV4 KO, and 20 of wild-type mice were used. (A, C) Nasal lavage and (B, D) blood. Horizontal lines indicate median values. The detection limit (the dotted line) is 666 CFU/ml. **p < 0.01.