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. 2021 Nov 3;12:732029. doi: 10.3389/fimmu.2021.732029

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Copyright © 2021 Kono, Nanushaj, Sakatani, Murakami, Hijiya, Kinoshita, Shiga, Kaneko, Enomoto, Sugita, Miyajima, Okada, Saika and Hotomi

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The phagocytic bactericidal ability of PMNs. PMNs isolated from each mouse strain were incubated with the bacteria (10² CFU) pre-opsonized with baby rabbit complement for 45 min in a bacterium: PMN ratio of 1:1000. The bacterial survival rate was calculated by dividing the number of bacteria in the experimental sample by those in the control sample that did not contain PMNs. The assay was triplicated and repeated three times for each mouse strain. Pooled data were displayed (N = 9 for each group). The mean value with the standard error of the mean was represented by a line bar. n.s., not significant, ****p < 0.0001.