| Composition | For 100 ml |
|---|---|
| 1.7 M Sucrose | 85 ml of 2 M solution |
| 10 mM Tris-HCl pH 8 | 1 ml of 1 M solution |
| 2 mM MgCl2 | 200 μl of 1 M solution |
| 0.15% Triton X-100 | 750 μl of 20% solution |
| 5 mM 2-mercaptoethanol | 35 μl of 14.4 M solution |
| Protease Inhibitors | 200 μl of 1 tablet/ml solution |
| H2O | 12.8 ml |
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