Composition	For 5 ml
50 mM Tris-HCl pH 8	250 μl of 1M solution
10 mM EDTA	100 μl of 0.5M solution
0.1% SDS	25 μl of 20% solution
Protease inhibitors	100 μl of 1 tablet/ml solution
H2O	4.525 ml