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. 2021 Nov 5;11(21):e4211. doi: 10.21769/BioProtoc.4211
Composition For 100 ml
1.1% Triton X-100 5.5 ml of 20% solution
1.2 mM EDTA 240 μl of 0.5 M solution
16.7 mM Tris-HCl pH 8 1.67 ml of 1 M solution
167 mM NaCl 3.34 ml of 5 M solution
Protease inhibitors 200 μl of 1 tablet/ml solution
H2O 89.05 ml