Composition | For 100 ml |
---|---|
1.1% Triton X-100 | 5.5 ml of 20% solution |
1.2 mM EDTA | 240 μl of 0.5 M solution |
16.7 mM Tris-HCl pH 8 | 1.67 ml of 1 M solution |
167 mM NaCl | 3.34 ml of 5 M solution |
Protease inhibitors | 200 μl of 1 tablet/ml solution |
H2O | 89.05 ml |