Composition	For 100 ml
150 mM NaCl	1.5 ml of 5M solution
0.1% SDS	250 μl of 20% solution
1% Triton X-100	2.5 ml of 20% solution
2 mM EDTA	200 μl of 0.5 M solution
20 mM Tris-HCl pH 8	1 ml of 1 M solution
H2O	44.3 ml