Figure 2. Agarose gel electrophoresis verification of DNA and RNA fragments for trLVPs generation.

(A) The genome sequence of trVLPs was divided into four fragments and each fragment PCR amplified. The purified PCR products of each fragment were determined by agarose gel. (B) PCR products of C-D-E and purified ligation products of full-length DNA (FL DNA). (C) Agarose gel analysis of SARS-CoV-2 N gene PCR products. (D) Agarose gel analysis of viral full-length RNA (FL-RNA) and (E) N gene mRNA generated by in vitro transcription using the FL DNA genome and N gene PCR products, respectively, as template. The black arrow indicates the FL RNA and N RNA, respectively.