Table 4. Thermal cycling program for PCR reaction to amplify fragment A-B.

Cycle step	Temperature	Time	Cycles
Initial denaturation	98°C	3 min	1
Denaturation	98°C	10 s	30
Annealing and extension	68°C	10 min
Final extension	68°C	10 min	1
	16°C	hold	-

Note: Usually, 30-40 μg of fragment A-B can be recovered from the 400 μl PCR reaction solution.