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| Component | Volume (μl) |
| 5× PrimeSTAR® GXL buffer | 10 |
| 2.5 mM dNTP | 4 |
| Forward primer THU3824 (10 μM) | 1 |
| Reverse primer THU3825 (10 μM) | 1 |
| Template (C-D-E ligation product) | 10 ng |
| PrimeSTAR® GXL | 1 |
| Deionized water | Up to 50 μl |
