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. 2021 Nov 5;11(21):e4249. doi: 10.21769/BioProtoc.4249

Figure 3. Neutralisation assays using SARS-CoV-2 pseudoparticles.

Figure 3.

SARS-CoV-2 Spike neutralisation assays were performed in the presence of (A) monoclonal antibodies (mAbs), presented as a percentage relative to untreated controls or (B) sera samples, with data expressed as neutralising titres. SARS-CoV-2 pseudoparticles were incubated with mAbs or sera for 1 h prior to addition of human ACE2-expressing HEK293T cells. Inhibition of SARS-CoV-2 Spike-mediated viral entry was determined by calculating the concentration (mAbs) or dilution (sera) at which there is a 50% reduction in luciferase signal (IC50). Data represent the mean ± SD of triplicate values, with each experiment performed three times on three separate occasions, and representative data shown.