Fig. 2.

Regeneration of plantlets from Nicotiana tabacum L. leaves transformed with pBI121 by biolistic transformation with silver nanoparticles (100 nm) at optimized conditions (900 psi He pressure and 6 cm target distance). A Control leaves in callus induction and selection media 3 days after bombardment with silver nanoparticles without pBI121. B Leaves in callus induction and selection media 7 days after bombardment with pBI121 coated silver nanoparticles. C Callus induction from leaf disc bombarded with pBI121 coated silver nanoparticles after 3 weeks. D Regeneration of plantlets from callus