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Stable transgene integration in regenerated plantlets checked by PCR using GUS gene-specific primers (Lane 1: 100 bp DNA marker; Lane 2: Positive control with pBI121 DNA used as PCR template; Lane 3–6: genomic DNA from regenerated plantlets transformed with gold microcarrier used as PCR template; Lane 7–10: genomic DNA from regenerated plantlets transformed with silver nanoparticles used as PCR template; Lane 11: non-template control
