Figure 2.

CD11b-deficient B cells exhibit impaired ability to resolve colitis. (A) Each Cd79a^−/− mouse intravenously received 1 × 10⁷ PP-derived B cells from WT mice (Itgam^+/+ ) or Itgam^−/− mice 2 days before the induction of colitis. Mice were evaluated daily, and weight loss (B) and disease activity index (DAI) scores (C) were recorded. (D) Representative distal colon histological sections of Cd79a^−/− mice were stained with hematoxylin and eosin (H&E), and the histological score was calculated. Images are displayed at the original magnification of ×100. Red stars indicate the position of the inflammatory cell infiltration and/or epithelial damage. (E) PP-derived B cells of WT mice were sorted and stimulated with lipopolysaccharide (LPS) for 48 h in vitro. CD11b⁺ and CD11b⁻ B cells were subsequently purified and intravenously injected (5 × 10⁶ cells per mice) into WT mice treated with DSS for 3 days. Weight loss (F) and the DAI scores (G) of recipient mice were measured and evaluated from day 0 to day 7. (H) H&E-stained histological sections of distal colon tissue are presented. Images are displayed at the original magnification of ×100. Red stars indicate the position of the histological injury. **P < 0.01, ***P < 0.001. Data are expressed as mean ± SEM of eight mice.