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. 2021 Nov 3;12:697725. doi: 10.3389/fimmu.2021.697725

Figure 4.

Figure 4

B cells expressing CD11b contribute to the differentiation of IgA in mice with colitis. (A) Expression levels of IgA in CD11b+ B cells and CD11b− B cells in the PPs and colorectal LP at days 0, 4, 7, and 10 after treatment with DSS were detected using flow cytometry. The frequency and absolute number of IgA-expressing cells are presented. (B) The adoptively transferred Itgam+/+ or Itgam−/− mice into Cd79a−/− mice were as described in Figure 1 . The percentage and absolute number of IgA+ B cells in the LP and PPs from DSS-treated Cd79a−/− mice were analyzed using flow cytometry. (C) The supernatant of the mucus-containing fluid was harvested and transferred into 10 mL phosphate-buffered saline (PBS), and the production of sIgA in the colon was determined using enzyme-linked immunosorbent assay (ELISA). (D) The adoptively transferred CD11b+ and CD11b− B cells into WT mice were as described in Figure 1 . (E) The supernatant of the mucus-containing fluid was harvested and transferred into 10 mL PBS, and the production of sIgA in the colon was determined using ELISA. **P < 0.01; ***P < 0.001. Data are expressed as mean ± SEM of six mice.