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AD2 influences both the ligand-dependent and intrinsic basal activities of rNRC.1. pBL-G5-CAT2 was cotransfected in HeLa cells with the Gal4 DBD and the two Gal4 fusions of rNRC.1 as depicted. The amino acids indicated for the rNRC.1s are those which are homologous to amino acids 771 to 864 and 771 to 1046 of hNRC. The ligands used are as indicated.
