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hNRC harbors a strong activation domain (AD1) and an inhibitory S-T-L region at the C terminus. The indicated Gal4 fusions of hNRC and rNRC.1 were transfected in HeLa cells with pBL-G5-CAT2. The amino acids indicated for the rNRC.1s are those which are homologous to amino acids 771 to 1046 of hNRC. The transfections were analyzed in duplicate, and the experiment was repeated at least three times with similar results. Gal4-VP16 was used as a control for comparison.
