FIG. 3.

Ligand-dependent interaction of rNRC.1 and hNRC with nuclear hormone receptors in yeast. (A) rNRC.1 was expressed as a B42 fusion protein and tested against LexA fusions of cTRα, hTRβ-LBD, hGR-LBD, hRARα-LBD, hRXRα-LBD, hERα, and hVDR-LBD. Both ligand-dependent and ligand-independent interactions were quantified by measuring the activity of β-galactosidase in yeast extracts. Details are given in Materials and Methods, along with the ligands used. (B) Same as panel A except that hNRC was expressed as a B42 fusion and examined for interaction with the various LexA-receptor chimeras.