FIG. 8.

Receptor binding induces a conformational change in hNRC. The hRXRα LBD was cloned into pJG4-6. This vector is similar to pJG4-5 but lacks the B42 activation domain. It includes the same HA epitope and same simian virus 40 viral nuclear localization signal as found in pJG4-5, which we introduced. RXR-pJG4-6 expressing the hRXRα LBD was cotransformed into yeast along with LexA-hNRC as indicated. pJG4-6 alone or with pEG202 (LexA), LexA-hRXR-LBD, or LexA-hNRC was transformed into yeast as controls. RXRΔAF2-pJG4-5, contains the hRXRα LBD AF2 mutation expressed as a fusion protein with the B42 activation domain. RXRΔAF2-pJG4-5 was cotransformed with LexA or LexA-hNRC as shown. Yeast cells were incubated with or without 9-cis-RA, and the activity of β-galactosidase was determined.