Table 1.

Values for Tim protein binding to vesicles composed of PO and DO phospholipids

Binding parameter	Tim1	Tim3	Tim4
EC50 for PO lipids, μM	57 (48, 66)	355 (277, 432)	45 (40, 51)
EC50 for DO lipids, μM	22 (19, 24)	92 (85, 100)	16 (14, 18)
b0 for PO lipids	0.03 (0.02, 0.05)	0 (0, 0.03)	0.07 (0.06, 0.08)
b0 for DO lipids	0.07 (0.05, 0.1)	0.03 (0.02, 0.04)	0.33 (0.31, 0.35)
bmax for PO lipids	0.90 (0.85, 0.95)	0.90 (0.85, 0.96)	0.69 (0.67, 0.71)
bmax for DO lipids	0.99 (0.97, 1.00)	1.00 (0.99, 1.00)	0.98 (0.96, 1.00)

Values were obtained by fitting data in Fig. 3, b–d to Eq. 1. Briefly, the vesicles contained 7:3 PC/PS, the fatty acyl chains of which were either PO or DO and total lipid concentration was 300 μM. Brackets give 95% confidence intervals. The values for each parameter are significantly different for the three Tim proteins at >95% confidence as calculated using a z test.