Table 2.

Values for the parameters describing Tim protein binding to PS-containing vesicles

Binding parameter	Tim1	Tim3	Tim4
Hill coefficient, $h$	1.38 (1.2, 1.57)∗	1.65 (1.59, 1.72)	2.28 (2.17, 2.39)∗
$K$ for Tim-Ca2+-PS dissociation, μM	61 (59, 63)	127 (120, 136)	35 (34, 36)
$K_{\mathit{TC}}$ for Tim-Ca2+ dissociation, μM	590 (540, 650)	4300 (3600, 5200)	136 (123, 150)
$K_{\mathit{TS}}$ for Tim-PS dissociation, μM	1800 (1300, 2700)	>5000	239 (220, 258)

Values for Tim3 were obtained by fitting the data in Fig. 4, b and c to Eq. S4. Values for Tim1 and Tim4 were obtained by fitting the data in Figs. S3 and S4 to Eq. 2, utilizing $h$-values recalculated from the primary data for Figure 5 of Tietjen et al. (14). Brackets give 95% confidence intervals. The values for each parameter are significantly different for the three Tim proteins at >95% confidence as calculated using a z test.

^∗The previously obtained values for the Hill coefficients of Tim1 and Tim4 were recalculated after reanalysis of the primary data using the updated data analysis methodology of this work; see Supporting materials and methods.