Figure 10.

Stabilization of the MAL-5′UTR dimer by neomycin. (A) Nondenaturing gel electrophoresis of the indicated RNAs in TB buffer. Samples were dimerized in PI buffer for 1 h at 37°C with 0–10 μM neomycin. Dimer (D) and monomer (M) species are indicated. (B) FCCS data displayed as in Fig. 3. MAL-5′UTR254 was dimerized as in (A) in the presence of 10 μM neomycin. The presence of neomycin was maintained during acquisition of FCCS data. (C) ES plot for the MAL-5′UTR²⁵⁴ dimer, formed and imaged in PI buffer at 1 mM Mg²⁺ with 10 μM neomycin and acquired with the intra-UTR FRET assay. (D) Inter-UTR FRET data for MAL-5′UTR²⁵⁴ dimerized and imaged in PI buffer with 1 mM Mg²⁺ and 10 μM neomycin. ES plots and univariate histograms are displayed as in Figs. 6 and 8. To see this figure in color, go online.