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. 2021 Sep 14;120(21):4874–4890. doi: 10.1016/j.bpj.2021.09.017

Figure 4.

Figure 4

Stability of NL4.3 and MAL genomic RNA dimers isolated from virions. (A) Nondenaturing Northern blot of viral RNA extracted from media of cells transfected with HIV-1 Ψ-helper and NL4.3 (lanes 1–7) or MAL (lanes 8–14) leader RNAs. RNA was incubated at indicated temperature for 5 min before electrophoresis. Migration of viral genomic RNA dimers (D) and monomers (M) are indicated. (B) Percentage of viral genomic RNA monomers at different incubation temperatures as quantified by phosphorimager. Quantifications of three independent experiments are presented, except temperatures 52 and 62°C, which show data from one blot only. Bars reflect the average and standard deviation. Statistical significance was determined using two-way analysis of variance and indicated with asterisk notation.