Figure 9.

Summary of the MAL-5′UTR²⁵⁴ conformation occupancies. The occupancies in the observed MAL-5′UTR²⁵⁴ conformations are displayed in pie charts. The results of all smFRET experiments shown in Figs. 8 and 10, performed with both the intra-UTR and inter-UTR assays, are shown. Conformations were identified according to FRET efficiency and S-value and are abbreviated as follows: KD, kissing dimer; ED, extended dimer; DCM, dimer-competent monomer. The occupancies were calculated as the number of FRET observations in high or low FRET, as indicated, divided by the total number of FRET observations and displayed as a percentage. In the intra-UTR assay, “Donor only” molecules arise from incomplete labeling of the RNA and from unannealed DNA handle. In the inter-UTR assay, the “Donor only” population arises additionally from 5′UTR monomers. smFRET trajectories that displayed intermediate-FRET values that are inconsistent with the identified conformations were not assigned (NA). These data are also presented in Table S1. To see this figure in color, go online.