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. Author manuscript; available in PMC: 2022 Apr 11.
Published in final edited form as: Nat Biotechnol. 2021 Oct 11;39(11):1385–1393. doi: 10.1038/s41587-021-01042-y

Extended Data Figure 5. Reduction of axial side-lobes by deconvolution and optimization of the 3D-iLLS PSF.

Extended Data Figure 5.

a, Reduction of side-lobes in 3D-iLLS imaging using deconvolution. Top: raw z profiles of two individual mRNA molecules, from the data in Figure 2a. Bottom: z profiles of the same mRNAs, after 10 iterations of the Richardson-Lucy deconvolution algorithm with an experimental PSF. b-i, Optimized 3D-iLLS PSF based on a fundamental rectangular 2D bound lattice. b, SLM pattern and c, corresponding intensity at rear pupil. d Annular mask. e, Intensity at rear pupil after annular mask. f, Resulting 2D bound lattice in real space and g, corresponding dithered lattice excitation pattern. h, Axial profile of conventional LLS PSFs. i, Axial profile of 3D-iLLS PSFs. Cyan: excitation; yellow: detection; gray: overall.