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. Author manuscript; available in PMC: 2022 Apr 11.
Published in final edited form as: Nat Biotechnol. 2021 Oct 11;39(11):1385–1393. doi: 10.1038/s41587-021-01042-y

Figure 3. 3D-iLLS-SIM enables cellular imaging with improved resolution compared to conventional LLS-SIM and dithered LLS.

Figure 3

a, Top and middle panels: maximum intensity projections of Atto 647N immunostained microtubules in a fixed COS-7 cell. Bottom panels: slice of the data in the plane indicated by the transparent yellow lines. b, Line profiles of individual microtubules in z. c, Line profiles of individual microtubules in x. b-c, Dashed lines: raw data; solid lines: 1D Gaussian peak fits. Fitted z FWHM widths are 122 nm, 311 nm, and 603 nm, for 3D-iLLS-SIM, and for conventional LLS-SIM and LLS (5-phase average), respectively. Fitted x FWHM widths are 216 nm and 401 nm, for 3D-iLLS-SIM and conventional LLS (5-phase average), respectively. Three experiments were repeated independently with similar results.