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. Author manuscript; available in PMC: 2022 Apr 11.
Published in final edited form as: Nat Biotechnol. 2021 Oct 11;39(11):1385–1393. doi: 10.1038/s41587-021-01042-y

Figure 4. 3D-iLLS-SIM enables two-color imaging with extended resolution.

Figure 4

a, Two-color imaging of mitochondria (Tomm-20 Atto647N) and microtubules (tubulin, CF568) in a fixed COS-7 using 3D-iLLS-SIM. Images show maximum intensity projections in the yx plane. b, Montage showing consecutive z sections (100 nm z-steps) through a single mitochondrion (boxed in a). c, Orthoslices and line profiles of the CF 568 immunostained mitochondrion shown in b. Dashed lines: raw data; solid lines: 1D Gaussian peak fits. Fitted FWHM is 110 nm, and 152 nm in z and 199 nm, and 206 nm in x, respectively. e, Orthoslice showing microtubule cross-sections in the zx plane. f, Line profiles (dashed lines) of the microtubules in e, and 1D Gaussian peak fits (solid lines). Fitted FWHM is 140 nm, 157 nm and 114 nm in z and 248 nm, 251 nm, and 225 nm in x, respectively. Two experiments were repeated independently with similar results.