FIGURE 2.

p4 limits experimental S. aureus infection in vivo. WT and ChemKO mice were treated with vehicle (PBS) or were topically infected with 1 × 10⁷ CFU of S. aureus 8325-4 in the presence of 100 μM p4, control peptide scp4, or PBS. (A) Data points indicate the number of bacteria recovered from the skin surface 3 h after application of bacteria. (B) Data points indicate the number of live bacteria recovered from the skin surface 24 h after application of bacteria. (C) Data points indicate Camp expression levels in the skin treated with PBS for 24 h. Each data point represents one cavity, and a horizontal line indicates the mean value in each group; n = 5–10 independent experiments (1–2 mice per group). ***p < 0.001; **p < 0.01; *p < 0.05 by the Kruskal–Wallis test with post hoc Dunn’s multiple comparisons test and one-way ANOVA with Tukey’s post hoc test.