FIG. 6.

Pulse-labeling analysis of general Pol II-mediated transcription in DT40-TAF31 cells. DT40-TAF31 cells were cultured in medium with or without Tet for the indicated times. Each culture was pulse labeled with [³H]uridine for 10 to 15 min. Total cellular RNAs were purified, and poly(A)⁺ RNA was selected on an oligo(dT) column. The ratios of selected poly(A)⁺ RNA to total RNA were determined after scintillation counting of equal aliquots of each sample. The ratios for control samples (cells grown in the absence of Tet [−]) were designated 100%, and the ratios for test samples (cells grown in the presence of Tet [+]) are presented relative to this. Results from three to five independent experiments for each of the indicated time points were averaged and plotted. Error bars represent standard deviation.