FIG. 8.
RNase protection analysis of serum-induced c-fos expression. Total cellular RNA was isolated from DT40-TAF31 cells incubated in the presence of Tet for the indicated times, with or without 10 h of serum starvation and 75 min of serum stimulation. Equal amounts of total RNAs for each sample were analyzed by RNase protection using c-fos, cTAFII31, or TBP probes, and the protected bands were separated on polyacrylamide gels.