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. 2021 Nov 16;12:6626. doi: 10.1038/s41467-021-26991-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a IDO activity assays (IDO1, 2 or 20 µM; H₂O₂ 2 µM; l-Trp; 100 µM) were carried out in the absence and presence of His-tagged Prx2 (4 µM). Reactions were incubated for 5 min at 25 °C and H₂O₂-derived Trp oxidation products (cis-WOOH and OIA) determined by LC-MS/MS. b Samples analyzed in (a) were collected and alkylated with NEM and probed by SDS-PAGE and western blotting for Prx2 oxidation state. Data are shown as mean ± SEM (n = 4 independent enzyme assays). Source data are provided as a Source data file.