Figure 19.

Peptide-based Zn²⁺ fluorescent probes. a) Increase in fluorophore emission due to binding-induced peptide conformational regulation. Protein scaffold shields the fluorophore from solvent interactions. b) Structure of the Palm-ZP1 (34), which was used for localized Zn detection in live HeLa cells. The peptide contains a 3-residue polyproline helix, 2 sequential Asp residues and a C-terminal Lys to serve as the point of attachment for the Zn²⁺ probe.