Table 2.
Positive effects of perlecan on biomaterials improvement.
| Species | Cell/tissue location | Modified biomaterials | Result | References |
|---|---|---|---|---|
| Human | MG-63 cell | Coating electrospun collagen and gelatin fibers with PlnDI | Increasing FGF-2 binding | [184] |
| BMSC/MG-63 cell | PlnDI-containing COL I scaffold | Promoting cell proliferation by enhancing FGF-2 activity | [204] | |
| C4-2B cell | Electrospun PCL-based scaffolds with PlnDIV | Facilitating cell proliferation, survival, and migration | [205] | |
| Coronary artery endothelial cell | Silk biomaterials functionalized with PlnDV | Supporting cell adhesion, spreading, and proliferation | [206] | |
| Mouse | Cartilage | PlnDI immobilized to HA microgel | Repairing OA-like damage by controlled release of BMP-2 | [113] |
| C3H10T1/2 cell | PlnDI-conjugated, HA-based HGPs | Stimulating chondrogenic specific ECM production by binding and sustained release of BMP-2 | [111] | |
| PlnDI and COL II coated PLA scaffold | Promoting cartilage-like tissue in PlnDI/COL II coated PLA scaffold | [141] | ||
| BMSC | PlnDI-functionalized electrospun PCL scaffolds | Enhancing ALP activity by increasing PCL loading capacity of BMP-2 | [207] | |
| RGC-5 cell | Biological glue made by mixing laminin, COL IV, entactin, and Pln | Improving retina adhesion rate | [208] | |
| Rat | Skin | PlnDI-containing chitosan scaffolds | Promoting angiogenesis and dermal wound healing | [159] |
| Bone | A recombinant PlnDI expressed from HEK 293 cells with HS/CS PG tightly binding recombinant human BMP-2 | Promoting dose-enhancement of BMP-2 on a particulate TCP scaffold to generate up to 9 fold more bone volume in 6 or more weeks in a rat model than the control without PlnDI | [112] |
Abbreviation: ALP: alkaline phosphatase; BMP-2: bone morphogenetic protein 2; BMSC: bone marrow stromal cell; C3H10T1/2: a murine mesenchymal stem cell line; C4-2B: a cancer cell line; CS: chondroitin sulfate; ECM: extracellular matrix; FGF-2: fibroblast growth factor 2; HA: hyaluronic acid; HGP: hydrogel particle; HS: heparan sulfate; MG63: an osteoblastic cell line; OA: osteoarthritis; PCL: poly (epsilon-caprolactone); PLA: polylactic acid; PG: proteoglycan; RGC-5: a retinal ganglion cell line; TCP: tricalcium phosphate