REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
rabbit polyclonal anti-L. monocytogenes R11 | Dramsi et al., 1998 | N/A |
mouse monoclonal anti-β-actin clone AC-15 | Sigma-Aldrich | Cat# A1978, RRID:AB_476692 |
rabbit monoclonal anti-c-Met | Abcam | Cat# ab51067, RRID:AB_880695 |
rabbit polyclonal anti-SURF1 | Proteintech | Cat# 15379-1-AP, RRID:AB_2239968 |
rabbit polyclonal anti-Rab11 | Invitrogen | Cat# 71-5300, RRID:AB_2533987 |
rabbit polyclonal anti-Transferrin receptor | Abcam | Cat# ab84036, RRID:AB_10673794 |
anti-rabbit Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-11034, RRID:AB_2576217 |
anti-rabbit Alexa Fluor 594 | Thermo Fisher Scientific | Cat# A-11012, RRID:AB_2534079 |
anti-rabbit Alexa Fluor 658 | Thermo Fisher Scientific | Cat# A-11011, RRID:AB_143157 |
Bacterial and virus strains | ||
Listeria monocytogenes EGDe (wt strain) | BUG1600, Institut Pasteur | N/A |
Listeria monocytogenes EGDeΔC3Δhpt (strain deleted by lmo1293, lmo0347/8, lmo2695/6 and uhpt) | Grubmüller et al., 2014 | N/A |
Listeria monocytogenes EGDe-cGFP (wt strain expressing green fluorescent protein) | BUG2538, Institut Pasteur | N/A |
Listeria monocytogenes EGD (wt strain) | BUG600, Institut Pasteur | N/A |
Listeria monocytogenes EGDΔInlA (InlA deleted strain) | BUG947, Institut Pasteur | N/A |
Listeria monocytogenes EGDΔInlB (InlB deleted strain) | BUG1047, Institut Pasteur | N/A |
Listeria monocytogenes EGDΔhly (hly deleted strain) | BUG3650, Institut Pasteur | N/A |
Listeria monocytogenes EGD-cGFP (wt strain expressing GFP) | BUG2539, Institut Pasteur | N/A |
Salmonella enterica serovar typhimurium 12023 GFP (wt strain expressing GFP) | Institut Pasteur | N/A |
Shigella flexneri M90T (wt strain) | Centre d’Immunologie de Marseille-Luminy | N/A |
Chemicals, peptides, and recombinant proteins | ||
Brain heart infusion (BHI) medium | BD Difco | Cat#237500 |
McCoy’s 5A medium (Modified) | GIBCO | Cat#26600 |
McCoy’s 5A medium (Modified) (costum-made based on #26600, GIBCO without glucose and glutamine) | GIBCO | Costum-made |
DMEM, no glucose | GIBCO | Cat#11966 |
D-(+)-Glucose | Sigma-Aldrich | Cat#G8270 |
D-(+)-Galactose | Sigma-Aldrich | Cat#G0625 |
L-glutamine | GIBCO | Cat#25030 |
Non-essential amino acids | GIBCO | Cat#11140 |
L-sodium pyruvate | GIBCO | Cat#11360 |
Fetal bovine serum (FBS) | BioWest | Cat#S181G |
[U-13C6]glucose (99% labeled) | Sigma-Aldrich | Cat#389374 |
[U-13C6]galactose (99% labeled) | Sigma-Aldrich | Cat#605379 |
[U-13C5]glutamine (98% labeled) | Cortecnet | Cat#CC1050P01 |
Gentamicin | Sigma-Aldrich | Cat#G1397 |
Penicillin-Streptomycin | GIBCO | Cat#15140-122 |
Chloramphenicol | Sigma-Aldrich | Cat#C0378 |
G418 | InvivoGen | Cat#108321-42-2 |
Triton X-100 (TX-100) | Sigma-Aldrich | Cat#11332481001 |
jetPRIME | Polypus Transfection | Cat#114-01 |
oligomycin A | Sigma-Aldrich | Cat#75351 |
carbonyl cyanide p-(tri-fluromethoxy)phenyl-hydrazone (FCCP) | Sigma-Aldrich | Cat#C2920 |
carbonyl cyanide m-chlorophenyl hydrazone (CCCP) | Sigma-Aldrich | Cat#C2759 |
rotenone | Sigma-Aldrich | Cat#R8875 |
antimycin A | Sigma-Aldrich | Cat#A8674 |
2-Deoxy-D-glucose (2DG) | Sigma-Aldrich | Cat#D6134 |
dynasore hydrate | Sigma-Aldrich | Cat#D7693 |
Phalloidin 647 | Thermo Fisher Scientific | Cat#A22287 |
MitoTracker Deep Red | Thermo Fisher Scientific | Cat#M22426 |
Hoechst 33342 | Thermo Fisher Scientific | Cat#62249 |
NucBlue Live ReadyProbes Reagent | Thermo Fisher Scientific | Cat#R37605 |
4–20% Mini-PROTEAN TGX Stain-Free Protein Gels | Bio-Rad | Cat#4568096 |
Critical commercial assays | ||
Protein Assay Dye Reagent Concentrate | Bio-Rad | Cat##5000006 |
Amersham ECL Prime | Thermo Fisher Scientific | Cat#10308449 |
Clarity Western ECL substrate | Bio-Rad | Cat# #1705060 |
ATPlite kit | Perkin-Elmer | Cat#6016943 |
LIVE/DEAD Fixable Red Dead Cell Staining dye | Thermo Fisher Scientific | Cat# L34971 |
Deposited data | ||
GC-MS data | this manuscript | XXX |
Experimental models: Cell lines | ||
Human: HCT116 wt | ATCC | CCL-247 |
Human: HCT116 SURF1−/− | this manuscript | N/A |
Human: HeLa | ATCC | CCL-2 |
Human: primary skin fibroblasts EF10 + tel | Tissue repository of the Association Française contre les Myopathies (AFM) | N/A |
Human: primary skin fibroblasts KB5 + tel | Tissue repository of the Association Française contre les Myopathies (AFM) | N/A |
Oligonucleotides | ||
SURF1 fwd (for CRISPR/Cas9 deletion of SURF1) 5′ caccgCGCCTGGAGGAGCGTCCTCA 3′ | this manuscript | N/A |
SURF1 rev (for CRISPR/Cas9 deletion of SURF1) 5′ aaacTGAGGACGCTCCTCC AGGCGc 3′ |
this manuscript | N/A |
SURF1 fwd (for complementation of SURF1−/− cells) 5′ gatccccaagctt ggcctgacaggccTCACAC ACCAGGTGTCCCAC 3′ |
this manuscript | N/A |
SURF1 rev (for complementation of SURF1−/− cells) 5′ actaccccaagc tggcctctgagTTGGTACCGAGC TCGGATC 3′ |
this manuscript | N/A |
Recombinant DNA | ||
Plasmid: pSpCas9(BB)-2A-GFP (PX458) | Ran et al., 2013 | Addgene plasmid #48138 |
Plasmid pSpCas9(BB)2A-GFP containing the sgRNA for SURF1 | this manuscript | N/A |
Plasmid: pcDNA3.1+C-(hSURF1)DYK | GenScript | CloneID OHu20778 |
Plasmid: pSBbi-Neo | Kowarz et al., 2015 | Addgene plasmid #60525 |
Plasmid pSBbi-Neo-SURF1 | this manuscript | N/A |
Plasmid: pCMV (CAT)T7-SB100 | Mátés et al., 2009 | Addgene plasmid #34879 |
Plasmid: pEGFP-N1 | Clontech Laboratories | #6085-1 |
Plasmid: pC1-EGFP-Rab11bCA | Dr. Matthew Lawrenz, University of Louisville | N/A |
Software and algorithms | ||
Prism (version 8) | GraphPad | RRID:SCR_002798 |
Seahorse Wave (version 2.6.1) | Agilent | RRID:SCR_014526 |
LabSolutions | Shimadzu | RRID:SCR_018241 |
MetaMorph (version 7.7.9.0) | Molecular Devices | RRID:SCR_002368 |
Fiji (version 2.0.0) | ImageJ | RRID:SCR_002285 |
Image Lab (version 6.0.1) | Bio-Rad | RRID:SCR_014210 |
FlowJo (version 10) | BD Biosciences | RRID:SCR_008520 |