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. 2021 Nov 9;37(6):109989. doi: 10.1016/j.celrep.2021.109989
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

rabbit polyclonal anti-L. monocytogenes R11 Dramsi et al., 1998 N/A
mouse monoclonal anti-β-actin clone AC-15 Sigma-Aldrich Cat# A1978, RRID:AB_476692
rabbit monoclonal anti-c-Met Abcam Cat# ab51067, RRID:AB_880695
rabbit polyclonal anti-SURF1 Proteintech Cat# 15379-1-AP, RRID:AB_2239968
rabbit polyclonal anti-Rab11 Invitrogen Cat# 71-5300, RRID:AB_2533987
rabbit polyclonal anti-Transferrin receptor Abcam Cat# ab84036, RRID:AB_10673794
anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific Cat# A-11034, RRID:AB_2576217
anti-rabbit Alexa Fluor 594 Thermo Fisher Scientific Cat# A-11012, RRID:AB_2534079
anti-rabbit Alexa Fluor 658 Thermo Fisher Scientific Cat# A-11011, RRID:AB_143157

Bacterial and virus strains

Listeria monocytogenes EGDe (wt strain) BUG1600, Institut Pasteur N/A
Listeria monocytogenes EGDeΔC3Δhpt (strain deleted by lmo1293, lmo0347/8, lmo2695/6 and uhpt) Grubmüller et al., 2014 N/A
Listeria monocytogenes EGDe-cGFP (wt strain expressing green fluorescent protein) BUG2538, Institut Pasteur N/A
Listeria monocytogenes EGD (wt strain) BUG600, Institut Pasteur N/A
Listeria monocytogenes EGDΔInlA (InlA deleted strain) BUG947, Institut Pasteur N/A
Listeria monocytogenes EGDΔInlB (InlB deleted strain) BUG1047, Institut Pasteur N/A
Listeria monocytogenes EGDΔhly (hly deleted strain) BUG3650, Institut Pasteur N/A
Listeria monocytogenes EGD-cGFP (wt strain expressing GFP) BUG2539, Institut Pasteur N/A
Salmonella enterica serovar typhimurium 12023 GFP (wt strain expressing GFP) Institut Pasteur N/A
Shigella flexneri M90T (wt strain) Centre d’Immunologie de Marseille-Luminy N/A

Chemicals, peptides, and recombinant proteins

Brain heart infusion (BHI) medium BD Difco Cat#237500
McCoy’s 5A medium (Modified) GIBCO Cat#26600
McCoy’s 5A medium (Modified) (costum-made based on #26600, GIBCO without glucose and glutamine) GIBCO Costum-made
DMEM, no glucose GIBCO Cat#11966
D-(+)-Glucose Sigma-Aldrich Cat#G8270
D-(+)-Galactose Sigma-Aldrich Cat#G0625
L-glutamine GIBCO Cat#25030
Non-essential amino acids GIBCO Cat#11140
L-sodium pyruvate GIBCO Cat#11360
Fetal bovine serum (FBS) BioWest Cat#S181G
[U-13C6]glucose (99% labeled) Sigma-Aldrich Cat#389374
[U-13C6]galactose (99% labeled) Sigma-Aldrich Cat#605379
[U-13C5]glutamine (98% labeled) Cortecnet Cat#CC1050P01
Gentamicin Sigma-Aldrich Cat#G1397
Penicillin-Streptomycin GIBCO Cat#15140-122
Chloramphenicol Sigma-Aldrich Cat#C0378
G418 InvivoGen Cat#108321-42-2
Triton X-100 (TX-100) Sigma-Aldrich Cat#11332481001
jetPRIME Polypus Transfection Cat#114-01
oligomycin A Sigma-Aldrich Cat#75351
carbonyl cyanide p-(tri-fluromethoxy)phenyl-hydrazone (FCCP) Sigma-Aldrich Cat#C2920
carbonyl cyanide m-chlorophenyl hydrazone (CCCP) Sigma-Aldrich Cat#C2759
rotenone Sigma-Aldrich Cat#R8875
antimycin A Sigma-Aldrich Cat#A8674
2-Deoxy-D-glucose (2DG) Sigma-Aldrich Cat#D6134
dynasore hydrate Sigma-Aldrich Cat#D7693
Phalloidin 647 Thermo Fisher Scientific Cat#A22287
MitoTracker Deep Red Thermo Fisher Scientific Cat#M22426
Hoechst 33342 Thermo Fisher Scientific Cat#62249
NucBlue Live ReadyProbes Reagent Thermo Fisher Scientific Cat#R37605
4–20% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Cat#4568096

Critical commercial assays

Protein Assay Dye Reagent Concentrate Bio-Rad Cat##5000006
Amersham ECL Prime Thermo Fisher Scientific Cat#10308449
Clarity Western ECL substrate Bio-Rad Cat# #1705060
ATPlite kit Perkin-Elmer Cat#6016943
LIVE/DEAD Fixable Red Dead Cell Staining dye Thermo Fisher Scientific Cat# L34971

Deposited data

GC-MS data this manuscript XXX

Experimental models: Cell lines

Human: HCT116 wt ATCC CCL-247
Human: HCT116 SURF1−/− this manuscript N/A
Human: HeLa ATCC CCL-2
Human: primary skin fibroblasts EF10 + tel Tissue repository of the Association Française contre les Myopathies (AFM) N/A
Human: primary skin fibroblasts KB5 + tel Tissue repository of the Association Française contre les Myopathies (AFM) N/A

Oligonucleotides

SURF1 fwd (for CRISPR/Cas9 deletion of SURF1) 5′ caccgCGCCTGGAGGAGCGTCCTCA 3′ this manuscript N/A
SURF1 rev (for CRISPR/Cas9 deletion of SURF1) 5′ aaacTGAGGACGCTCCTCC
AGGCGc 3′
this manuscript N/A
SURF1 fwd (for complementation of SURF1−/− cells) 5′ gatccccaagctt
ggcctgacaggccTCACAC
ACCAGGTGTCCCAC 3′
this manuscript N/A
SURF1 rev (for complementation of SURF1−/− cells) 5′ actaccccaagc
tggcctctgagTTGGTACCGAGC
TCGGATC 3′
this manuscript N/A
Recombinant DNA
Plasmid: pSpCas9(BB)-2A-GFP (PX458) Ran et al., 2013 Addgene plasmid #48138
Plasmid pSpCas9(BB)2A-GFP containing the sgRNA for SURF1 this manuscript N/A
Plasmid: pcDNA3.1+C-(hSURF1)DYK GenScript CloneID OHu20778
Plasmid: pSBbi-Neo Kowarz et al., 2015 Addgene plasmid #60525
Plasmid pSBbi-Neo-SURF1 this manuscript N/A
Plasmid: pCMV (CAT)T7-SB100 Mátés et al., 2009 Addgene plasmid #34879
Plasmid: pEGFP-N1 Clontech Laboratories #6085-1
Plasmid: pC1-EGFP-Rab11bCA Dr. Matthew Lawrenz, University of Louisville N/A

Software and algorithms

Prism (version 8) GraphPad RRID:SCR_002798
Seahorse Wave (version 2.6.1) Agilent RRID:SCR_014526
LabSolutions Shimadzu RRID:SCR_018241
MetaMorph (version 7.7.9.0) Molecular Devices RRID:SCR_002368
Fiji (version 2.0.0) ImageJ RRID:SCR_002285
Image Lab (version 6.0.1) Bio-Rad RRID:SCR_014210
FlowJo (version 10) BD Biosciences RRID:SCR_008520