Figure 3.

Dsn1 itself is found in a gradient at anaphase kinetochores

(A) HeLa cells enriched in anaphases by thymidine release were stained for DNA (blue), Dsn1S109ph (green), and CENP-C (red).

(B) HeLa cells as in (A) stained for DNA (blue), total Dsn1 (green), and CENP-C (red).

(C) Quantification of Dsn1S109ph (11 cells) and total Dsn1 (15 cells) at individual kinetochores as a function of distance from the midzone. Dots are shaded as in Figure 1D. Using linear regression, for Dsn1S109ph, slope = −6.5 and is non-zero (p < 0.0001, F test). For total Dsn1, slope = −2.1 and is non-zero (p < 0.0001, F test). The slopes are significantly different from one another (p < 0.0001, F test). Confidence intervals (95%) are shown as fine lines.

Scale bars: 5 μm (A and B). See also Figure S3.