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. 2021 Nov 16;11:22340. doi: 10.1038/s41598-021-01752-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Functional results of deorphanized TAS2Rs (TAS2Rs) response to their representative bitter compounds, comparing with empty pcDNA vector as a negative control. The TAS2R DNAs, G16-gust44, and GCaMP calcium sensor were co-expressed in HEK293E cells. The fluorescence signal changes were measured by adding representative bitter compounds to the corresponding TAS2Rs, using a FlexStation 3 microplate reader (96 well plate).