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. 2021 Nov 16;7:357. doi: 10.1038/s41420-021-00750-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A UBE2S protein levels in five HCC cell lines were determined by western blot. B–E Cytotoxicity curves show the cytotoxic effects of 5-FU (B) and oxaliplatin (D) on five different HCC cell lines. Bar graphs (C, E) show the IC50 values of n = 3 independent experiments. F UBE2S knockdown and overexpression rescue were validated by western blot in Huh7 and PLC/PRF/5 cells, respectively. G–J Cytotoxic effects of 5-FU on UBE2S-knockdown and UBE2S-rescue (knockdown + overexpression) Huh7 (G, H) and PLC/PRF/5 (I, J) cells and control (NTC + Vec) cells were determined by CCK-8 assays, respectively. K–N Cytotoxic effects of oxaliplatin on UBE2S-knockdown and UBE2S-rescue Huh7 (K, L) and PLC/PRF/5 (M, N) cells and control cells were determined, respectively. O UBE2S overexpression efficiency was validated by western blot in Hep3B and HepG2 cells, respectively. P–S Cytotoxic effects of 5-FU on UBE2S-overexpression Hep3B (P, Q) and HepG2 (R and S) cells and vector control (Vec) cells were determined by CCK-8 assays, respectively. T–W Cytotoxic effects of oxaliplatin on UBE2S-overexpression Hep3B (T, U) and HepG2 (V, W) cells and Vec cells were determined, respectively.