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. 2021 Nov 16;12:6624. doi: 10.1038/s41467-021-26912-6

Fig. 5. 1D8 interferes with cell fusion and binding.

Fig. 5

Quality control of gH/gL expression on the surface of CHO-K1 cells by staining with 1D8 (a), positive control AMMO1 (b), and negative control 2G4 (c) before proceeded to the fusion experiment shown in (d). d Marked reduction in cell-cell fusion in the presence of 1D8, the positive control AMMMO1, but not the negative control 2G4. Marked reduction in gH/gL binding to Raji B cell (e) and HK1 epithelial cell (f) in the presence of 1D8, the positive control AMMO1, but not the negative control 2G4. Binding of EphA1-Fc to gH/gL was reduced by 1D8 (g), but not by AMMO1 (h) or 2G4 (i) measured by BLI. All data are presented as mean ± SEM from three replicates. *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significant; two-tailed unpaired Student’s t test. d ***p < 0.0001; (e) ***p < 0.0001; (f) gp42 **p = 0.0057, AMMO1 *p = 0.0478, 1D8 *p = 0.010. SSC-A, side-scatter area; PC5.5, PerCP-Cy5.5; RLU, relative light unit; SA-PE, streptavidin-phycoerythrin; MFI, mean fluorescence intensity. Source data are provided as a Source Data File.